Protein microinjection by protease permeabilization of fibroblasts

Protein microinjection by protease permeabilization of fibroblasts.

Exposure of cultured diploid fibroblasts to protease solutions induces a hyperpermeable state which permits entry of exogenous macromolecules directly into the cytosol. We have exploited this finding to devise a microinjection method whose chief advantages are simplicity and good retention of cell viability. Proteins successfully injected by this technique range from insulin to thyroglobulin. T...

Microinjection of the ras oncogene protein into nonestablished rat embryo fibroblasts.

Early (twice) and late (eighth) passage nonestablished rat embryo fibroblasts exhibit dramatically different responses to microinjection of the oncogenic form (T24) of the ras protein. Late passage cells respond like established cultures in that they both synthesize DNA and divide in response to the oncogenic protein. However, early passage cells are shown to be much less responsive to the intr...

Xenopus oocyte microinjection: from gene to protein.

Protein processing by the placental protease , cathepsin

Cathepsin P is a member of a family of placentally expressed cathepsins (PECs). The closest human homolog of cathepsin P is cathepsin L, a broad specificity enzyme that has functions in many tissues in addition to placenta. The gene duplications that gave rise to the PECs provide a rare opportunity to define proteolytic functions in placenta, a transient organ unique to mammals. Peptidyl substr...

control of the optical properties of nanoparticles by laser fields

در این پایان نامه، درهمتنیدگی بین یک سیستم نقطه کوانتومی دوگانه(مولکول نقطه کوانتومی) و میدان مورد مطالعه قرار گرفته است. از آنتروپی ون نیومن به عنوان ابزاری برای بررسی درهمتنیدگی بین اتم و میدان استفاده شده و تاثیر پارامترهای مختلف، نظیر تونل زنی(که توسط تغییر ولتاژ ایجاد می شود)، شدت میدان و نسبت دو گسیل خودبخودی بر رفتار درجه درهمتنیدگی سیستم بررسی شده اشت.با تغییر هر یک از این پارامترها، در...